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By Nathan P. Kaplan, William B. Jakoby, Meir Wilchek

The severely acclaimed laboratory usual, tools in Enzymology, is likely one of the so much hugely revered courses within the box of biochemistry. on account that 1955, each one quantity has been eagerly awaited, often consulted, and praised by way of researchers and reviewers alike. The sequence includes a lot fabric nonetheless correct this day - actually an important ebook for researchers in all fields of lifestyles sciences.

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Hay, I. Strachan, and G. A. Levvy, Biochem. J. 102, 929 (1967). t It. L. Lai and B. Axelrod, Biochem. Biophys. Res. Commun. 64, 463 (1973). B. E. Evans and R. Wolfenden, J. Am. Chem. Soc. 92, 4751 (1970). P. W. K. Woo, It. W. Dion, S. M. Lange, L. F. Dahl, and L. J. Durham, J. Helerocycl. Chem. 11, 641 (1974). R. M. Cohen and R. Wolfenden, J. Biol. Chem. 246, 7561 (1971). L. D. Byers and R. Wolfenden, J. Biol. Chem. 247, 606 (1972). A. Schmitt, I. Bottke, and G. Siebert, Hoppe-Seyler's Z. Physiol.

13 1, T. C. French, I. B. Dawid, R. A. Day, and J. M. Buchanan, J. Biol. Chem. 238, 217 (1963) ; I. B. Dawid, T. C. French, and J. M. Buchanan, ibid. 238, 2186 (1963). See also this volume [45]. 13R. tIandschumacher, C. Bates, P. Chang, A. Andrews, and G. Fisher, Proc. Am. Assoc. Cancer Res. 8, 25 (1967). See also this volume [47]. 36 GENERAL METHODOLOGY ~] DONV does not affect this enzyme. This is as expected, since DONV should not be a substrate for the enzyme. Both azaserine and DON irreversibly inhibit formylglycinamide ribotide amidotransferase.

However, if reaction conditions are altered, bromopyruvate may act as an affinity label of aspartate aminotransferase. Recent experiments of [4] SYNCATALYTIC ENZYME MODIFICATION 47 Morino and collaborators is have indicated that a residue other than Cys390 is affinity labeled when the normal dicarboxylic amino acid cosubstrate is replaced by alanine in the presence of formate. Similarly ambiguous behavior of an affinity label might also arise if its mere binding to the enzyme were to induce an increase in reactivity of enzyme side chains.

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Affinity Labeling: Affinity Labeling by Nathan P. Kaplan, William B. Jakoby, Meir Wilchek

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